Ọnụ ọgụgụ njikọ njikọ otu ọwa | 5noml-5umol. |
Oge okirikiri synthesis | ruo 6-8 nkeji |
okirikiri synthesis (20mer) | 2-3 awa |
Ihe ndabere karama phosphoramite | 8 setịpụ |
Ntọala karama reagent | 7 setịpụ |
Kalama isi | 60/240/480ml ịghasa ọnụ eluigwe na ala reagent kalama interface |
Kalama inyeaka reagent | Ọnụ karama GL38, jiri maka karama reagent 4L zuru ụwa ọnụ. |
Usoro ịnya ụgbọ ala reagent | ihe nchebe gas ala mgbali ụdị |
Mwepu mmiri na-ekpofu | ezigbo nrụgide |
Ọnụọgụ njikọ | 99% |
Ogologo kachasị elu | gafere 120mer |
Ịnye ọkụ | Otu-adọ 220V. |
Ọnọdụ okpomọkụ na-arụ ọrụ | 20C°± 5C° |
Iru mmiri dị ala | n'ime 40% |
Nkwụsi ike nke ọrụ ahụ | Ọ nwere ike na-arụ ọrụ nke ọma ma na-aga n'ihu. |
Nyochaa | LCD |
Akwụkwọ ikike | 1 Afọ |
1. Onye ọ bụla reagent bụ ọwa nọọrọ onwe ya site na karama nchekwa mmiri mmiri na kọlụm njikọ na-enweghị ọwa ndị ọzọ.
2. A na-agbakwunye activator na Phosphoramidite n'usoro n'usoro n'oge usoro njikọ na premixed na kọlụm njikọ iji mee mmeghachi omume ahụ.
3. A kwadebere ya na efere abụọ nwere ngụkọta nke kọlụm njikọ 192 na ọdụ ụgbọ mmiri 12-20 isi.
4. Na mgbakwunye na 4 ọkọlọtọ bases na sịntetik auxiliary reagents, e nwekwara 8 modified bases, nke nwere ike synthesized dị ka chọrọ, dị ka thiomodification, ma ọ bụ ndị ọzọ fluorescent mgbanwe na abụọ-labeled TAQMAN probes, wdg Karịa 8 pụrụ iche bases.
5. Ọ nwere njikọ na-akpaghị aka na nchịkọta nke ntọala na-enweghị mkpa maka tupu mixing nke Phosphoramidites.
6. N'oge usoro nhazi dum, enwere ụfọdụ ego na ụlọ mmepụta ihe (ọnụego gas nwere ike gbanwee) A na-ejupụta gas nchebe iji gbochie ikuku ịbanye n'ime oghere njikọ ahụ ma na-emetụta àgwà njikọ.
7. Reagents na Phosphoramite nwere ike kwajuru n'ime karama abụọ n'otu oge, nke nwere ike dịkwuo irè oge nke otu njikọ na-enweghị ugboro ugboro nnọchi nke reagents.
8. Ejiri ya na valvụ mgba ọkụ na-ehicha ihe, na acetonitrile na argon nwere ike iji kpochapu pipeline na valves iji zere mgbochi nke pipeline na valves kpatara site na njedebe ogologo oge nke ngwá ọrụ.
9. Akụrụngwa nwere ọrụ nke nyocha onwe ya na nchebe, gbanyụọ ọkụ, kwụsịtụ ma gaa n'ihu.
10. Ọnọdụ ọrụ na ihe nchekwa chọrọ kwekọrọ n'ụkpụrụ ma ọ bụ ụkpụrụ China na mba ụwa.