Akụrụngwa dị ọcha nke oligo maka njikọ primer Oligo

Akụrụngwa dị ọcha nke oligo maka njikọ nke Oligo primer,
igwe nkwado oligo synthesis,

Nkọwapụta

Ụzọ dị iche iche nke ịdị ọcha

1. Gel Electrophoresis Chromatography dị ọcha
Jiri denaturing polyacrylamide gel electrophoresis chromatography maka ịdị ọcha.Onye na-ahụ maka denaturing bụ n'ozuzu 4M formamide ma ọ bụ 7M urea, ịta nke acrylamide dị n'etiti 5-15%, na oke nke methacrylamide bụ tumadi n'etiti 2-10%.
Mgbe electrophoresis gasịrị, a ga-ekpebi ọnọdụ nke eriri nucleic acid n'okpuru ọkụ ultraviolet, gel nke nwere ebumnuche nucleic acid na-ebipụ, a na-akụjikwa nucleic acid ma leachara, wee tinye ihe ngwọta leaching, gbanye ya. ọnụọgụ na lyophilized.

2. DMT-On, HPLC dị ọcha
Họrọ ụdị DMT-On n'oge njikọta ahụ, etinyere ngwaahịa crude ma tinye ya n'ime ụlọ okpomọkụ iji wepụ oke amonia mgbe aminolysis gasịrị.
Emere nkewa site na iji kọlụm C18 nwere acetonitrile na 10% triethylamine-acetic acid (TEAA) dị ka eluent.Mgbe emechara elution ahụ, a na-etinye uche ya, wee wepụ ìgwè DMT na trifluoroacetic acid.Mgbe nnọpụiche ahụ gasịrị, a na-ewepụ ụfọdụ nnu na obere ụmụ irighiri ihe site na tube bepụ, ma mechaa gbanye ya.

Usoro a nwere ike nweta ngwaahịa nwere ịdị ọcha dị elu, ma ọ dị mkpa ịṅa ntị na ihe omume nke depurination.

3. DMT- Gbanyụọ, HPLC Ọcha
Họrọ DMT-Off n'oge njikọ, na ngwaahịa crude bụ centrifuged na lekwasị na ụlọ okpomọkụ iji wepụ amonia ngafe mgbe ammonolysis.
Emere nkewa site na iji kọlụm C18 nwere acetonitrile na 10% triethylamine-acetic acid na mmiri dị ka eluent.Mgbe emechara nkewa ahụ na ọnụ ọgụgụ, a na-eme ka aliquots lyophilized.

Usoro a na-achọ nhazi nke ọma nke ọnọdụ nkewa, ma nwee ike nwetakwa mkpụrụ ndụ ebumpụta ụwa dị ọcha.

oligo synthesis accerry equipment for dna rna synthes cycle, which suit for normal primer, and other short chain primer, na-eme ka oligo di ocha.
onye na-emepụta china nke oligo synthesis equipment with good quality and best service.