Ọnyà molekụla maka phosphoramite na reagents egosipụtara

Ọnyà molekụla maka phosphoramidite na reagents

Ngwa:

A na-eji ọnyà molecular na-eme ka mmiri dị n'ime reagents na amidite mee ya, na mbụ e mere ya maka njikọ nke oligonucleotides.Ọ dabara adaba, enweghị uzuzu na enweghị flannel.Enwere ike ịgbakwunye ya na ihe mgbaze dị iche iche na ihe ngwọta organic iji wepụ mmiri mmiri.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa ngwaahịa

Na ABI na MilliGen / PerSeptive DNA synthesis tests, sub-sieve mkpọ nwere ike idobe ọdịnaya mmiri na nitrile na karama activator n'okpuru 10 ppm, na-enweghị nchegbu banyere valvụ ma ọ bụ valvụ trotul na-ekewa n'ime sub-sieve.Uzuzu ma ọ bụ lint dị na akpa nkwakọba kpuchiri.Enwere ike iji ya mee ihe ozugbo maka mmiri mmiri nke 500 ml, 1L, 2 L na karama mgbaze ndị ọzọ, na nkọwapụta mkpọ sub-sieve nwekwara ike ịhazi ya.

Ihe na-esonụ na-egosi mmetụta mmịpụta dị ike nke ọnyà molekụla 10 g na 500ml, 1L, 4L nitrile samples nwere mmiri dị iche iche.

Tụlee ọnyà molekụla mbubata

500 ml 197 ppm ACN
Oge (h)	0	24	48	72	96
HonyaBio	197	33	16.5	6.5	6
Mba ndị ọzọ	197	43	27	15	15

1 L 143 ppm ACN
Oge (h)	0	24	48	72	96
HonyaBio	143	48	32	20	15
Mba ndị ọzọ	142	47	36	23	15

4 L 141 ppm ACN
Oge (h)	0	24	48	72	96
HonyaBio	141	95	94	84	73
Mba ndị ọzọ	141	96	95	85	72

Ntuziaka na ojiji

A na-ejupụta ọnyà molekụla ahụ, ọ dịkwa mkpa ka e meghee ya ọbụlagodi na ejiri ya, a ga-echekwa akara nke karama reagent mgbe ejiri ya.
2g sieve maka awa 24 nwere ike ibelata ọdịnaya mmiri 165 ppm na 500 ml nitrile na 105 ppm.
5 g sieve maka awa 24 nwere ike ibelata ọdịnaya mmiri 172 ppm na 500 ml nitrile na 58 ppm.
10 g sieve 24 h Ọ nwere ike ibelata ọdịnaya mmiri 166 ppm na 1 L nitrile na 68 ppm.
20 g sub-sieve nwere ike ibelata ọdịnaya mmiri 162 ppm na 4 L nitrile na 109 ppm maka awa 24.

Anyị na-akwado 2 g subsieve maka 50-250ml karama reagent, 5g maka 250-500ml reagent karama, 10g maka 500-1000ml reagent karama, na 20g maka 1000-2000ml reagent karama.

Nkwenye

Ngwungwu obere ihuenyo ọ bụla na-enweta njikwa mma siri ike n'oge usoro mmepụta.Ma ọ dị mkpa ka ndị ahịa lelee nkwakọ ngwaahịa tupu ejiri ya:

Nke mbụ, gosi na oghere nkwakọ ngwaahịa adịghị.Mwepu oghere ọ bụla ma ọ bụ ntinye ikuku ga-ebelata arụmọrụ nke ngwaahịa ahụ.

Nke abụọ, kpachara anya mgbe ị na-emepe nkwakọ ngwaahịa iji gbochie ihe nkiri nkwakọ ngwaahịa dị n'okpuru ihuenyo, wee lelee ma ihe nkiri ahụ ọ̀ dị.

Ọgwụgwọ mgbe emechara ya

Ọ bụghị ihe na-egbu egbu ma ọ bụ na-emerụ ahụ, a ga-emerụ ha site na kemịkalụ na ihe mgbaze ha na-abanye na kọntaktị na ekwesịrị ikpofu ha dị ka ihe mkpofu emeru emeru mgbe ejiri ya.

Ụdị na ngwa

Anyị na mbụ nyere sub-sieve akpa na nha nke 2 g, 5 g, 10 g, na 20 g., na ndị ọzọ nkọwa nwere ike ahaziri dị ka ndị ahịa mkpa.

Ọnyà molekụla dabara adaba maka ikpughe ogologo oge na nitrile, a pụkwara iji ya mee ihe maka acetic acid, ether, acetic ether, butyl acetate, mmanya, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, nitric acid, phosphoric acid. , sulfuric acid, methyl chloride, nitrogen methyl imidazole, wdg.

O nwere ike ọ gaghị adị mma maka ikpughe ogologo oge na tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) na ngwọta ndị ọzọ.