A na-eme njikọ njikọ nucleic acid site na iji usoro siri ike sialic amide triglyceride, nke a na-eme ka njedebe 3 nke DNA ghara imegharị ya na mkpụrụ osisi siri ike na nucleotides na-agbakwunye na 3' ruo 5' direction ruo mgbe a na-ejikọta iberibe DNA achọrọ. .Nke a dị iche na njikọ DNA site na ntinye nke DNA polymerase.
A na-eme ka njedebe nke 3 nke mbụ dị na CPG n'oge njikọ, 5'-OH nke isi na-esote na-echebe ya na di-p-tolyl trityl DMT, amino group na isi na-echebe ya na benzoic acid na 3′ -OH na-arụ ọrụ na amino phosphate compound.1 isi nke 5 5 ′-OH nke 1 isi na 3′-OH nke na-esote isi na-etolite a phosphite triglyceride, nke na-ahụ oxidised na ayodiin ka a phosphate triglyceride, onye na-echebe na nke abụọ isi 5′-OH wepụrụ site na. na-agbakwunye dichloroacetic acid DM cycles site na mgbakwunye nke isi na-esote, na mgbe njikọta ihe nchebe na 5′-OH na-ewepụ ya na acid na-adịghị ike A na-ewepụ iberibe ahụ site na resin siri ike na ammonium hydroxide na-etinye uche, a na-ewepụ ihe nchebe na isi. na ammonium hydroxide na-etinye uche n'okpuru kpo oku, a na-ewepụ ammonium hydroxide, a na-ekpochapụ iberibe ahụ, a na-ehichapụkwa nucleic acid site na chromatography mmiri ma ọ bụ PAGE.
Nzọụkwụ Oligo Synthesis
Mgbochi
NkeA na-ewepụ 5 end DMT otu nchekwa site na ịgbakwunye ngwọta TCA.
Nkwalite
Ịgwakọta onye na-eme ihe na oligonucleotide monomer ka ọ bụrụ etiti oligonucleotide monomer na-arụ ọrụ.
Njikọta
Otu 5-terminal hydroxyl na-emeghachi omume na etiti oligonucleotide na-arụ ọrụ na mmeghachi omume condensation nke na-eme ka ọ bụrụ njikọ phosphite triglyceride na-adịghị akwụsi ike.
Nkwudo okwu
Mgbakwunye nke onye na-ahụ maka capping na-eme mmeghachi omume capping acetylation na oke hydroxyl dị iche iche na-adịghị etinye aka na mmeghachi omume condensation.
Oxidation
Mgbakwunye nke ngwọta ayodiin nwere mmiri na-emeghachi omume na njikọ phosphite na-adịghị akwụsi ike site na oxidation iji mepụta njikọ phosphoric acid triglyceride kwụsiri ike.
Cyanoethyl Cleavage
Ngwaahịa nucleic acid mgbe njikọta, yana mgbakwunye nke ngwọta DEA iji wepụ otu cyanoethyl na njikọ phosphate triglyceride.
Ekewara&nchekwa
A na-agbaji iberibe acid nucleic site na onye na-ebu akụkụ siri ike site na ịgbakwunye ngwọta ammonium hydroxide na-ekpo ọkụ wee kpoo ọkụ iji wepụ otu ndị na-echebe site na njikọ hydrogen nke bases.
Oge nzipu: Nov-21-2022