Enwere ike ịhazi ụlọ ọrụ ọtụtụ ọrụ
Akụkụ Elution
1. Ahịrị nke oghere injection mmiri 8, nke nwere ike ịgbanye n'otu oge, na isi mmiri mmiri nwere ike ịchịkwa n'otu n'otu.
2. 5 reagent karama ọnọdụ, nke ọ bụla karama na-onwem na 4L reagent karama, ya bụ, ọ nwere ike dakọtara na 5 ụdị reagents.
3. A na-ebugharị ngwá ọrụ ahụ site na mmiri mmiri nrụgide dị mma, nke dị mma ma na-agbanwe agbanwe iji chịkwaa ọnụego mmiri mmiri.
4. A na-eji ngwá ọrụ ahụ mee ihe na ọkwa 96 nke ọma, na onye ahịa na-akọwapụta ụdị efere ahụ.
5. Enwere ike itinye efere 96 nke ọma nke ọma n'okpuru efere ahụ iji kpokọta mmiri mmiri, ma ọ bụ na-ejikọta ya na pipeline iji wepụ mmiri mmiri na-ekpofu ihe.
6. N'ime akụkụ ngwanrọ, ị nwere ike ịhọrọ ụdị reagent na ọnụ ọgụgụ nke reagent iji gbakwunye ihe nlele, enwere ike ịtọ oge, enwere ike ịtọ mgbatị nrụgide, oge nrụgide nwere ike ịchịkwa, na usoro ndị ahụ nwere ike ịtọ. na-agba ọsọ na-aga n'ihu.2 ụdị Ezi ntụziaka.
Akụkụ dị ọcha
1. Akụrụngwa nwere ike jupụta na ogidi 96 c18 n'otu oge.
2. A na-eji ngwá ọrụ ọkọlọtọ nwere ọdụ ụgbọ mmiri ise reagent, onye na-ejide tube centrifuge, na onye na-ejide ya nwere ike iburu 96 primer centrifuge tubes.
3. Akụrụngwa na-eji usoro nchịkwa nke onwe nke atọ-axis, na-enwe oke nkenke na ezigbo repeatability.
4. The akụrụngwa adopts mmiri nzacha ụdị.
5. Ntọala ngwanrọ na-agbanwe ma dị mfe iji rụọ ọrụ.
6. The mmiri isi na-onwem na mmiri mmiri larịị mmetụta ngwaọrụ na-akpaghị aka na-aghọta na mmiri mmiri larịị ịdị elu.
Akụkụ ọkpọkọ
1. 96-nke ọma efere ka centrifuge tube.
2. Ntụpọ pipette akpaaka: enwere ike kewaa primer ọ bụla n'ime ibe 2 n'ọtụtụ.
3. Mmegharị axis X, Y, Z.
4. Oke nke ụda mmiri (5-200ul).
5. Mgbe ị na-ekewa n'ime ọtụtụ tubes.
6. Oge chọrọ.
7. Enwere ike jikọta ya na template nha, Error ozugbo ọrụ, enwere ọrụ nkwụsịtụ n'etiti Red na-egosi na mkpokọta olu ezughị ezu ma ọ dị mkpa ka ekpuchiri ya.
Enwere ndụmọdụ na-acha uhie uhie, odo na akwụkwọ ndụ akwụkwọ ndụ maka ịgbakọ olu: odo pụtara na olu dị ntakịrị, ị nwere ike iji aka gbanwee olu ahụ, ọ dịghị mkpa ka ị na-agbanye, akwụkwọ ndụ akwụkwọ ndụ pụtara nkịtị.
8. A na-egosipụta ozi dịka nọmba kọlụm, ọnụ ọgụgụ tubes, na olu nke tube ọ bụla mgbe ị na-ejuputa ihe nlele ọ bụla.Ọkwa ahụ na-egosi na ekewala primers na ekewaghị ya, ndozi ọnọdụ akpaka tupu ekesa nke ọ bụla.
