Akụrụngwa dị ọcha maka ịdị ọcha Oligo
Nkọwapụta
| bọọdụ dakọtara | 1, 3, 5, 8. |
| nzacha | ịfụ filtration, imi filtration |
| Ọnụọgụ ọdụ ụgbọ mmiri injection | 5, 6, 7, 8, 9, 10. |
| Ụdị efere dakọtara | efere C18, efere miri emi nke ọma, efere sịntetik (dakọtara na ọtụtụ efere sịntetik), efere microtiter |
| Modul | otu axis ma ọ bụ abụọ axis |
| Voltaji | 220V |
| Akwụkwọ ikike | 1 afọ |
| Omenala | anabatara |
Ụzọ dị iche iche nke ịdị ọcha
1. Gel Electrophoresis Chromatography dị ọcha
Jiri denaturing polyacrylamide gel electrophoresis chromatography maka ịdị ọcha.Onye na-ahụ maka denaturing bụ n'ozuzu 4M formamide ma ọ bụ 7M urea, ịta nke acrylamide dị n'etiti 5-15%, na oke nke methacrylamide bụ tumadi n'etiti 2-10%.
Mgbe electrophoresis gasịrị, a ga-ekpebi ọnọdụ nke eriri nucleic acid n'okpuru ọkụ ultraviolet, gel nke nwere ebumnuche nucleic acid na-ebipụ, a na-akụjikwa nucleic acid ma leachara, wee tinye ihe ngwọta leaching, gbanye ya. ọnụọgụ na lyophilized.
2. DMT-On, HPLC dị ọcha
Họrọ ụdị DMT-On n'oge njikọta ahụ, etinyere ngwaahịa crude ma tinye ya n'ime ụlọ okpomọkụ iji wepụ oke amonia mgbe aminolysis gasịrị.
Emere nkewa site na iji kọlụm C18 nwere acetonitrile na 10% triethylamine-acetic acid (TEAA) dị ka eluent.Mgbe emechara elution ahụ, a na-etinye uche ya, wee wepụ ìgwè DMT na trifluoroacetic acid.Mgbe nnọpụiche ahụ gasịrị, a na-ewepụ ụfọdụ nnu na obere ụmụ irighiri ihe site na tube bepụ, ma mechaa gbanye ya.
Usoro a nwere ike nweta ngwaahịa nwere ịdị ọcha dị elu, ma ọ dị mkpa ịṅa ntị na ihe omume nke depurination.
3. DMT- Gbanyụọ, HPLC Ọcha
Họrọ DMT-Off n'oge njikọ, na ngwaahịa crude bụ centrifuged na lekwasị na ụlọ okpomọkụ iji wepụ amonia ngafe mgbe ammonolysis.
Emere nkewa site na iji kọlụm C18 nwere acetonitrile na 10% triethylamine-acetic acid na mmiri dị ka eluent.Mgbe emechara nkewa ahụ na ọnụ ọgụgụ, a na-eme ka aliquots lyophilized.
Usoro a na-achọ nhazi nke ọma nke ọnọdụ nkewa, ma nwee ike nwetakwa mkpụrụ ndụ ebumpụta ụwa dị ọcha.
