Ma DNA na RNA njikọ dabere na usoro nhazi usoro siri ike na kemịkalụ phosphoramidite, a pụrụ iji DNA synthesizer mee ka RNA ma ọ bụ RNA analogues na-enweghị mgbanwe ọzọ, na reagents na DNA njikọ nwere ike iji ozugbo na RNA na artificial nucleic acids. 'njikọ.Dị ka e gosiri na Figure 1, 2'-hydroxy na RNA phosphoramite na-echebe site na silyl nchebe otu, ya bụ t-butyldimethylsilyl (TBDMS), na-egbochi mmeghachi omume akụkụ na 2'-hydroxy otu na-esighi ike.Ndị otu TBDMS buru ibu gbochiri mmeghachi omume n'etiti phosphoramidite na otu 5'-hydroxy na nkwado siri ike, yana ogologo oge ijikọ ọnụ ka achọrọ iji nweta afọ ojuju arụmọrụ njikọ.
N'otu aka ahụ dị ka DNA njikọ, nke synthesized RNA bu ụzọ kpọwaa site na nkwado siri ike site na aminolysis, mgbe ahụ TBDMS otu tetrabutylammonium fluoride (TBAF) ma ọ bụ trimethylamine trihydrofluoride mebiri.Enwere ike sachaa RNA crude site na mmanya na-egbu egbu na HPLC.
Onyonyo 1. Nhazi kemịkalụ nke isi ihe ụlọ na DNA na njikọ RNA.
a) dABz phosphoramidite na b) rABz 2'-OTBDMS phosphoramidite.
Mmepe nke ọgwụ siRNA chọrọ analogues nhazi nke RNA nke ala iji mee ka bioacompatibility dị, 2'-hydroxy na RNA nwere ike dochie MeO, F na MOE otu, yana nucleic acid akpọchiri (LNA) na-enyekwa arụmọrụ dị mma na ọgwụgwọ RNA. (Foto nke 2).Ndị a phosphoramidites na-enye ọrụ ndị yiri ya dị ka DNA ụdị phosphoramidites na njikọ, na-arụ ọrụ na usoro dị ọcha nke ndị a na-abụghị eke nucleic acid yiri nke ala DNA.
Ọgụgụ 2. Usoro kemịkalụ nke ihe mgbochi ụlọ na ọgwụ siRNA.a) dABz 2-MeO phosphoramite;b) dABz 2-F phosphoramite;c) dABz 2-MOE phosphoramidite na d) dABz phosphoramidite akpọchiri.
Phosphoramiditesdị mkpa maka njikọ nke mkpụrụ ndụ ihe nketa, gụnyere tumadi DNA na ezinụlọ RNA na usoro ha, phosphoramite niile dị n'elu anyị nwere ike ịnye na ngwugwu.
Anyị na-emeziwanye arụmọrụ nke ngwaahịa anyị mgbe niile, na-emezi usoro mmepụta anyị ma nweta nkọwa ziri ezi.Ọ bụghị naanị na anyị na-enye gị ngwaahịa dị elu, kamakwa na-enye gị ọzụzụ na ọrụ.Anyị na-onwem na ọkachamara management mkpara na magburu onwe teknuzu otu na mmezi ọrụ na-enye ndị ahịa anyị zuru okè mgbe-sales ọrụ.
Oge nzipu: Ọgọst-09-2022